Jun-Bo Xue, Yong Huang, Jin-Ting Che, Rui Wang, Mei-Ke Liu and Wen-Kai Wang

Ephestia elutella, the tobacco moth, is regarded as a serious pest in the storage. E. elutella at low temperatures is conducive to parasitoid reproduction. Identifying the genes that respond to low temperature has important guiding significance for the breeding of parasitoid wasps. Transcriptome sequencing analysis of last instar E. elutella at normal and low temperature using RNA-seq. This study found that the expression of several genes in E. elutella changed significantly under low temperature. Among them, larvae cuticle protein, alkaline phosphatase and apyrase were significantly up-regulated, while Hsp70 and Hsp90 were significantly down-regulated. GO functional analysis showed that the genes may be involved in structural constituent of cuticle, catalytic activity, and metabolic process. KEGG functional analysis showed that the genes may be involved in glycolysis/gluconeogenesis and pentose phosphate pathway. These genes and pathways form the basis for subsequent breeding of parasitoid wasps and provide a reference for studying the mechanism of insect response to low temperature.

DOI: 10.22271/23940522.2024.v11.i2a.1018

Pages: 33-41  |  190 Views  89 Downloads